Managing Bacteria in a
Brewery
Even with using best practices to
ensure sanitary equipment, the yeast may harbor an infection which
grows with each re-pitching. Acid washing the yeast lowers the amount
of bacteria passed from batch to batch. Brewers most often use acid
washing as an emergency procedure to combat infection until fresh
yeast can be obtained or as periodic maintenance. Some brewers wash
every pitch to ensure absolutely every measure is taken to reduce
bacteria in the brewing process. Unfortunately information regarding
this practice is often conflicting or confusing.
How Acid Washing Works
Acid washing will lower the level of
bacteria cohabitation with yeast. It will not rid an infection. We
acidify the yeast slurry while keeping a low temperature to kill a
large proportion of bacteria and weak yeast cells. Healthy, actively
dividing and fermenting yeast produce an acidic environment
naturally. We can utilize the ability of healthy yeast to withstand
pH fluctuations to reduce the bacteria. Killing off weak and dying
yeast cells is also beneficial because they excrete peroxides and
autolysis factors which raises the pH of the total slurry and may
contribute off flavors to beer.
Acid washing after several pitches can
prevent a buildup of bacteria and weak yeast, allowing the healthy
yeast to replicate and ferment uninhibited by bacteria and autolysis
factors. Pediococcus affects yeast health by depressing yeast in
suspension by up to 30% and increasing fermentation length while also
producing high levels of diacetyl.
Acid washing has the potential to
negatively impact yeast health if performed inappropriately or on
certain strains of yeast. Time, pH, and temperature must be balanced
to achieve the goals of washing while maintaining highly viable yeast
for pitching.
Recommended Procedures
Overall goal:
Reduce yeast slurry pH to 2.0-2.4
range with food grade acid by constantly mixing at temperature range
of 32-40ºF
for 90 minutes.
Equipment:
Sterile recirculating reservoir with
pump or sterile mixing reservoir and paddle.
75% food grade phosphoric acid.
Ability to cool or at least hold cool
temperature.
Instructions:
Take initial yeast pH and temperature
readings.
Dilute 75% phosphoric acid to 7.5% by
a 1 to 10 dilution. The amount of 7.5% phosphoric acid required
will depend on volume of yeast. We used approximately 40L (10 gal)
per 100 gallons of yeast.
Start mixing yeast by paddle or
recirculation pump.
Add 7.5% phosphoric acid using sterile
technique (sanitize all connections)
Mix for 2-3 minutes and check pH and
temperature.
Adjust pH by adding more 7.5%
phosphoric acid or more water or yeast slurry
once pH range of 2.0-2.4 has been
achieved. Start timer. Wash for 90 minutes. Maintain temperature
between 32-40ºF.
After
90 minutes pitch yeast as soon as possible. Maximum storage for
washed yeast is two hours





